MWI(0) | 28 | kDa |
MWexpected | 27 | kDa |
VPorod | 45 | nm3 |
log I(s)
1.80×103
1.80×102
1.80×101
1.80×100
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Synchrotron SAXS
data from solutions of
TIP5 in Tris
in
20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH 8
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 3.2 and 7.2 mg/ml were measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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