| MWI(0) | 28 | kDa |
| MWexpected | 27 | kDa |
| VPorod | 45 | nm3 |
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log I(s)
1.80×103
1.80×102
1.80×101
1.80×100
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s, nm-1
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Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.
Tallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A, Structure 23(1):80-92 (2015) Europe PMCSASDA46 – TIP5 in Tris
Bromodomain adjacent to zinc finger domain protein 2A|
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Fits and models
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Synchrotron SAXS
data from solutions of
TIP5 in Tris
in
20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH 8
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 3.2 and 7.2 mg/ml were measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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