Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.

Tallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A, Structure 23(1):80-92 (2015) Europe PMC

SASDA56 – BAZ2B in Tris

Bromodomain adjacent to zinc finger domain protein 2B, C-terminal
MWI(0) 33 kDa
MWexpected 28 kDa
VPorod 43 nm3
log I(s) 2.80×101 2.80×100 2.80×10-1 2.80×10-2
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal small angle scattering data  s, nm-1
ln I(s)
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal Guinier plot ln 2.80×101 Rg: 4 nm 0 (4 nm)-2 s2
(sRg)2I(s)/I(0)
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal Kratky plot 1.104 0 3 sRg
p(r)
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal pair distance distribution function Rg: 4.2 nm 0 Dmax: 14.5 nm

Data validation


Fits and models


log I(s)
 s, nm-1
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal EOM/RANCH model

Synchrotron SAXS data from solutions of BAZ2B in Tris in 20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH 8 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Tags: X33
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal (BAZ2B)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   27.7 kDa
Sequence   FASTA