Dimerization-Induced Allosteric Changes of the Oxyanion-Hole Loop Activate the Pseudorabies Virus Assemblin pUL26N, a Herpesvirus Serine Protease.

Zühlsdorf M, Werten S, Klupp BG, Palm GJ, Mettenleiter TC, Hinrichs W, PLoS Pathog 11(7):e1005045 (2015) Europe PMC

SASDA58 – UL26N of pseudorabies virus

VP24
MWexperimental 45 kDa
MWexpected 53 kDa
VPorod 73 nm3
log I(s) 2.96×103 2.96×102 2.96×101 2.96×100
VP24 small angle scattering data  s, nm-1
ln I(s)
VP24 Guinier plot ln 2.96×103 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
VP24 Kratky plot 1.104 0 √3 sRg
p(r)
VP24 pair distance distribution function Rg: 2.7 nm 0 Dmax: 10.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
VP24 PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
VP24 DAMMIN model
Synchrotron SAXS data from solutions of UL26N of pseudorabies virus in 50 mM Tris/HCl 0.5 M NaCl 0.25 M imidazole 5% glycerol 50 mM urea 0.2 M MgCl2, pH 7.5 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 9.83 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

VP24 (UL26N)
Mol. type   Protein
Organism   Suid herpesvirus 1
Olig. state   Dimer
Mon. MW   26.6 kDa
 
UniProt   Q83417 (1-224)
Sequence   FASTA
 
PDB ID   4V07