RAID3--An interleukin-6 receptor-binding aptamer with post-selective modification-resistant affinity.

Mittelberger F, Meyer C, Waetzig GH, Zacharias M, Valentini E, Svergun DI, Berg K, Lorenzen I, Grötzinger J, Rose-John S, Hahn U, RNA Biol 12(9):1043-53 (2015) Europe PMC

SASDA88 – RAID3 in PBS

RAID3
MWexperimental 26 kDa
MWexpected 23 kDa
VPorod 26 nm3
log I(s) 2.00×10-3 2.00×10-4 2.00×10-5 2.00×10-6
RAID3 small angle scattering data  s, nm-1
ln I(s)
RAID3 Guinier plot ln 2×10-3 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
RAID3 Kratky plot 1.104 0 √3 sRg
p(r)
RAID3 pair distance distribution function Rg: 2.7 nm 0 Dmax: 10 nm

Data validation

Fits and models

log I(s)
 s, nm-1
RAID3 DAMMIN model
log I(s)
 s, nm-1
RAID3 SASREF CV model
Synchrotron SAXS data from solutions of RAID3 in PBS in PBS 137 mM NaCl 2,7 mM KCl 6,5 mM Na2HPO4 1,5 mM KH2PO4, pH 7.4 were collected on the B21 beam line at the Diamond Light Source storage ring (Didcot, UK) using a Pilatus 2M detector at a sample-detector distance of 3.9 m and (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.20 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN. Storage temperature = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN

RAID3
Mol. type   RNA
Olig. state   Dimer
Mon. MW   11.3 kDa
Sequence   FASTA