Structural basis of Deerpox virus-mediated inhibition of apoptosis.

Burton DR, Caria S, Marshall B, Barry M, Kvansakul M, Acta Crystallogr D Biol Crystallogr 71(Pt 8):1593-603 (2015) Europe PMC

SASDAD8 – Antiapoptotic membrane protein, (DpV84gp022) Deerpox virus

Antiapoptotic membrane protein
MWexperimental 39 kDa
MWexpected 39 kDa
VPorod 61 nm3
log I(s) 1.88×10-1 1.88×10-2 1.88×10-3 1.88×10-4
Antiapoptotic membrane protein small angle scattering data  s, nm-1
ln I(s)
Antiapoptotic membrane protein Guinier plot ln 1.88×10-1 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Antiapoptotic membrane protein Kratky plot 1.104 0 √3 sRg
p(r)
Antiapoptotic membrane protein pair distance distribution function Rg: 2.8 nm 0 Dmax: 11.1 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Antiapoptotic membrane protein, (DpV84gp022) Deerpox virus in 25 mM HEPES 150 mM NaCl, pH 7.5 were collected on the SAXS/WAXS beam line at the Australian Synchrotron storage ring (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.2 and 6.3 mg/ml were measured . 30 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Cell temperature = UNKNOWN

Antiapoptotic membrane protein
Mol. type   Protein
Organism   Deerpox virus W-1170-84
Olig. state   Dimer
Mon. MW   19.6 kDa
 
UniProt   Q08FF8 (1-155)
Sequence   FASTA