| MWI(0) | 23 | kDa |
| MWexpected | 22 | kDa |
| VPorod | 42 | nm3 |
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log I(s)
5.20×100
5.20×10-1
5.20×10-2
5.20×10-3
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s, nm-1
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Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy.
Xu X, Reinle W, Hannemann F, Konarev PV, Svergun DI, Bernhardt R, Ubbink M, J Am Chem Soc 130(20):6395-403 (2008) Europe PMCSASDAN5 – Cross-linked complex CytC_Adr
Cytochrome CAdrenodoxin
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Fits and models
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Synchrotron SAXS
data from solutions of
Cross-linked complex CytC_Adr
in
20 mM HEPES 2 mM DTT, pH 7.4
were collected
on the
EMBL X33 beam line
at the DORIS III, DESY storage ring
(Hamburg, Germany)
using a MAR 345 Image Plate detector
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 2.4 and 24 mg/ml were measured
at 15°C.
Two successive
120 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
Wavelength = UNKNOWN. Sample detector distance = UNKNOWN
Tags:
X33
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