MWexperimental | 45 | kDa |
MWexpected | 48 | kDa |
VPorod | 86 | nm3 |
log I(s)
4.34×101
4.34×100
4.34×10-1
4.34×10-2
|
![]() |
|
|
|
![]() |
|
![]() |
Synchrotron SAXS
data from solutions of
I27-PimA
in
50 mM Tris-HCl 150 mM NaCl, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.4 and 2.5 mg/ml were measured
at 10°C.
50 successive
1 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
|
|