| MWI(0) | 19 | kDa |
| MWexpected | 23 | kDa |
| VPorod | 32 | nm3 |
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log I(s)
5.90×102
5.90×101
5.90×100
5.90×10-1
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s, nm-1
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Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4.
Alonso-García N, García-Rubio I, Manso JA, Buey RM, Urien H, Sonnenberg A, Jeschke G, de Pereda JM, Acta Crystallogr D Biol Crystallogr 71(Pt 4):969-85 (2015) Europe PMCSASDAT6 – Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains
Integrin beta-4|
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Fits and models
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Synchrotron SAXS
data from solutions of
Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains
in
20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.5 and 48.6 mg/ml were measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
Combined use of SAXS, crystal structures, site directed spin labeling, double electron-electron resonance (DEER), and molecular modeling to analyze the structure of FnIII-3,4. |
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