| MWexperimental | 108 | kDa |
| MWexpected | 101 | kDa |
| VPorod | 154 | nm3 |
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log I(s)
1.69×104
1.69×103
1.69×102
1.69×101
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s, nm-1
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Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells.
Berneking L, Schnapp M, Rumm A, Trasak C, Ruckdeschel K, Alawi M, Grundhoff A, Kikhney AG, Koch-Nolte F, Buck F, Perbandt M, Betzel C, Svergun DI, Hentschke M, Aepfelbacher M, PLoS Pathog 12(6):e1005660 (2016) Europe PMCSASDAU8 – Immunosuppressive virulence protein YopM
Yersinia outer protein M (34-481)|
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Fits and models
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Synchrotron SAXS
data from solutions of
Immunosuppressive virulence protein YopM
in
50 mM HEPES 150 mM NaCl, pH 7.5
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.7 and 10.4 mg/ml were measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
YopM_34-481 is a dimer in solution |
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