The Structure of the Plakin Domain of Plectin Reveals an Extended Rod-like Shape.

Ortega E, Manso JA, Buey RM, Carballido AM, Carabias A, Sonnenberg A, de Pereda JM, J Biol Chem 291(36):18643-62 (2016) Europe PMC

SASDBC4 – Plakin domain of Human plectin (spectrin repeats: SR3-SR9)

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9
MWexperimental 93 kDa
MWexpected 96 kDa
VPorod 135 nm3
log I(s) 2.78×103 2.78×102 2.78×101 2.78×100
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 small angle scattering data  s, nm-1
ln I(s)
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 Guinier plot ln 2.79×103 Rg: 8.5 nm 0 (8.5 nm)-2 s2
(sRg)2I(s)/I(0)
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 Kratky plot 1.104 0 √3 sRg
p(r)
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 pair distance distribution function Rg: 9.1 nm 0 Dmax: 35 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 DAMMIF model
Synchrotron SAXS data from solutions of the plakin domain from Human plectin in 20 mM sodium phosphate, 150 mM NaCl, 5% v/v glycerol, 3 mM DTT were collected on the P12 beam line of Petra-III (Hamburg, Germany) using a Pilatus 2M detector (I(s) vs s; s = 4π sin θ/λ, where 2θ is the scattering angle and λ=0.124 nm). Different solute concentrations in the range 0.92-14.70 mg/ml were measured using an exposure time of 1 s (recorded as 20 x 0.050 s frames). The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering from the matched solvent-blank was subtracted. The data were normalized to protein concentration and then extrapolated to infinite dilution to simulate a zero-concentration scattering curve.

Plakin domain fragment of Human plectin encompassing spectrin repeats SR3-SR9 (Plectin)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   95.6 kDa
 
UniProt   Q15149-2 (543-1372)
Sequence   FASTA