| MWexperimental | 80 | kDa |
| MWexpected | 80 | kDa |
| VPorod | 114 | nm3 |
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log I(s)
3.48×10-2
3.48×10-3
3.48×10-4
3.48×10-5
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s, nm-1
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Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Shah N, Beckham SA, Wilce JA, Wilce MCJ, Nucleic Acids Res 46(6):3169-3186 (2018) Europe PMCSASDBG8 – Probable ATP-dependent RNA helicase DDX58 without CARDs (Delta-CARDs RIG-I)
Probable ATP-dependent RNA helicase DDX58 (without CARDs)|
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Fits and models
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Synchrotron SAXS data from solutions of Probable ATP-dependent RNA helicase DDX58 without CARDs (Delta-CARDs RIG-I) in 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH 7.4 were collected using size exclusion chromatography (SEC-SAXS) on the SAXS/WAXS camera on the storage ring Australian Synchrotron (Melbourne, Australia) using a Pilatus 1M detector at a sample-detector distance of 1.6 m and at a wavelength of λ = 0.10332 nm (I(s) vs s, where s = 4π sin θ/λ and 2θ is the scattering angle). Data frames (13 x 2 s exposures) were collected through the SEC elution peak at 34.4 min (GE Healthcare Life Sciences Superdex 200 10/300; 0.4 ml/min; 100 μl injection at 8 mg/ml). The data were normalized to the intensity of the transmitted beam and radially averaged; the radially averaged scattering of the solvent-blank was subtracted and the different curves were scaled to yield the final composite scattering curve. The SAXS data were collected at 15 °C.
Concentration min = UNKNOWN. Concentration max = UNKNOWN |
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