| MWI(0) | 48 | kDa |
| MWexpected | 51 | kDa |
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log I(s)
4.79×101
4.79×100
4.79×10-1
4.79×10-2
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s, nm-1
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Wing phosphorylation is a major functional determinant of the Lrs14-type biofilm and motility regulator AbfR1 in Sulfolobus acidocaldarius.
Li L, Banerjee A, Bischof LF, Maklad HR, Hoffmann L, Henche AL, Veliz F, Bildl W, Schulte U, Orell A, Essen LO, Peeters E, Albers SV, Mol Microbiol 105(5):777-793 (2017) Europe PMCSASDBJ2 – Wild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDNA-Sa oligonucleotide.
40bp long dsDNA-Sa OligonucleotideWild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family).
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Fits and models
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X-ray synchrotron radiation scattering data from solutions of N terminal affinity tag cleaved (by thrombin) wild type AbfR1 in complex with SaOligo in 0.5 x TBE buffer was collected on the BM29 beam line on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). A single concentration of 3.5mg/ mL was measured in 10 successive 20 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted .The analysis was performed using the ATSAS package. The displayed model is the best fit model with the lowest reduced Chi-squared value (1.55) after imposing P2 symmetry. The DNA is considerably bent in complex with ABfR1.
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