Structural characterization of the N-terminal part of the MERS-CoV nucleocapsid by X-ray diffraction and small-angle X-ray scattering.

Papageorgiou N, Lichière J, Baklouti A, Ferron F, Sévajol M, Canard B, Coutard B, Acta Crystallogr D Struct Biol 72(Pt 2):192-202 (2016) Europe PMC

SASDBQ3 – Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164)

Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164)
MWexperimental 24 kDa
MWexpected 18 kDa
VPorod 28 nm3
log I(s) 2.65×101 2.65×100 2.65×10-1 2.65×10-2
Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) small angle scattering data  s, nm-1
ln I(s)
Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) Guinier plot ln 2.65×101 Rg: 2.0 nm 0 (2.0 nm)-2 s2
(sRg)2I(s)/I(0)
Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) Kratky plot 1.104 0 √3 sRg
p(r)
Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) pair distance distribution function Rg: 2.0 nm 0 Dmax: 8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) DAMMIF model
log I(s)
 s, nm-1
Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) EOM/RANCH model
Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) EOM/RANCH model
Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) EOM/RANCH model
X-ray synchrotron radiation scattering data from solutions of Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) in 10 mM HEPES 300 mM NaCl were collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Different solute concentrations in the range 0.21-3.06 mg/ml were measured. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration. The low angle data collected were merged with the highest concentration high angle data to yield the final composite scattering curve. The three models represent the results obtained from ensemble optimisation method (EOM) analysis.

Middle East Respiratory Syndrome (MERS) coronavirus nucleocapsid N-Protein (N-terminal domain 1-164) (MERS N-Protein)
Mol. type   Protein
Organism   Middle East respiratory syndrome coronavirus
Olig. state   Monomer
Mon. MW   17.8 kDa
 
UniProt   T2B9R0 (1-164)
Sequence   FASTA
 
PDB ID   4UD1