Solution structure of the Atg1 complex: implications for the architecture of the phagophore assembly site.

Köfinger J, Ragusa MJ, Lee IH, Hummer G, Hurley JH, Structure 23(5):809-818 (2015) Europe PMC

SASDC73 – Atg1-Atg13-Atg17-Atg31-Atg29 Minipentamer Complex

Serine/threonine-protein kinase ATG1
Autophagy-related protein 13
Autophagy-related protein 17
Autophagy-related protein 29
KLLA0A10637p
MWexperimental 234 kDa
MWexpected 231 kDa
VPorod 1000 nm3
log I(s) 9.67×102 9.67×101 9.67×100 9.67×10-1
Serine/threonine-protein kinase ATG1 Autophagy-related protein 13 Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p small angle scattering data  s, nm-1
ln I(s)
Serine/threonine-protein kinase ATG1 Autophagy-related protein 13 Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p Guinier plot ln 9.67×102 Rg: 10.3 nm 0 (10.3 nm)-2 s2
(sRg)2I(s)/I(0)
Serine/threonine-protein kinase ATG1 Autophagy-related protein 13 Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p Kratky plot 1.104 0 3 sRg
p(r)
Serine/threonine-protein kinase ATG1 Autophagy-related protein 13 Autophagy-related protein 17 Autophagy-related protein 29 KLLA0A10637p pair distance distribution function Rg: 10.0 nm 0 Dmax: 34 nm

Data validation


There are no models related to this curve.

Synchrotron SAXS data from solutions of Atg1-Atg13-Atg17-Atg31-Atg29 Minipentamer Complex in 20 mM Tris, 200 mM NaCl, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.7 and 2.6 mg/ml were measured at 22°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN. Number of frames = UNKNOWN

Serine/threonine-protein kinase ATG1 (Atg1)
Mol. type   Protein
Organism   Kluyveromyces lactis
Olig. state   Dimer
Mon. MW   30.7 kDa
 
UniProt   Q6CSX2 (562-831)
Sequence   FASTA
 
Autophagy-related protein 13 (Atg13)
Mol. type   Protein
Organism   Kluyveromyces lactis
Olig. state   Dimer
Mon. MW   8.7 kDa
 
UniProt   Q6CWK2 (400-475)
Sequence   FASTA
 
Autophagy-related protein 17 (Atg17)
Mol. type   Protein
Organism   Kluyveromyces lactis
Olig. state   Dimer
Mon. MW   49.9 kDa
 
UniProt   Q6CS99 (1-423)
Sequence   FASTA
 
Autophagy-related protein 29
Mol. type   Protein
Organism   Kluyveromyces lactis
Olig. state   Dimer
Mon. MW   10.0 kDa
 
UniProt   Q6CTU8 (1-85)
Sequence   FASTA
 
KLLA0A10637p (Atg31)
Mol. type   Protein
Organism   Kluyveromyces lactis
Olig. state   Dimer
Mon. MW   16.1 kDa
 
UniProt   Q6CX74 (1-143)
Sequence   FASTA