A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover.

Deyaert E, Wauters L, Guaitoli G, Konijnenberg A, Leemans M, Terheyden S, Petrovic A, Gallardo R, Nederveen-Schippers LM, Athanasopoulos PS, Pots H, Van Haastert PJM, Sobott F, Gloeckner CJ, Efremov R, Kortholt A, Versées W, Nat Commun 8(1):1008 (2017) Europe PMC

SASDCG2 – Rab family protein CtRoco L487A

Rab family protein
MWexperimental 255 kDa
MWexpected 254 kDa
VPorod 447 nm3
log I(s) 1.02×102 1.02×101 1.02×100 1.02×10-1
Rab family protein small angle scattering data  s, nm-1
ln I(s)
Rab family protein Guinier plot ln 1.03×102 Rg: 5.0 nm 0 (5.0 nm)-2 s2
(sRg)2I(s)/I(0)
Rab family protein Kratky plot 1.104 0 √3 sRg
p(r)
Rab family protein pair distance distribution function Rg: 5.0 nm 0 Dmax: 18.4 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Rab family protein CtRoco L487A in 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT, pH 7.5 were collected on the BM29 camera on the storage ring ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). Inline SEC-SAXS was employed at 25°C (50 µl injection volume of an 8 mg/ml protein solution on a Bio SEC-3 HPLC column (Agilent, 3 µm 300 Å)). 51 successive frames were collected through the elution peak. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled and averaged to produce the final SAXS data displayed in this entry.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN

Rab family protein (CtRoco)
Mol. type   Protein
Organism   Chlorobaculum tepidum
Olig. state   Dimer
Mon. MW   127.1 kDa
 
UniProt   Q8KC98 (1-1102)
Sequence   FASTA