The vaccinia virus DNA polymerase structure provides insights into the mode of processivity factor binding.

Tarbouriech N, Ducournau C, Hutin S, Mas PJ, Man P, Forest E, Hart DJ, Peyrefitte CN, Burmeister WP, Iseni F, Nat Commun 8(1):1455 (2017) Europe PMC

SASDCP5 – Vaccinia virus DNA polymerase processivity factor component A20 (C-terminal fragment)

DNA polymerase processivity factor component A20 C-ter fragment
MWexperimental 19 kDa
MWexpected 17 kDa
VPorod 31 nm3
log I(s) 5.59×100 5.59×10-1 5.59×10-2 5.59×10-3
DNA polymerase processivity factor component A20 C-ter fragment small angle scattering data  s, nm-1
ln I(s)
DNA polymerase processivity factor component A20 C-ter fragment Guinier plot ln 5.59×100 Rg: 2.2 nm 0 (2.2 nm)-2 s2
(sRg)2I(s)/I(0)
DNA polymerase processivity factor component A20 C-ter fragment Kratky plot 1.104 0 √3 sRg
p(r)
DNA polymerase processivity factor component A20 C-ter fragment pair distance distribution function Rg: 2.2 nm 0 Dmax: 7.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
DNA polymerase processivity factor component A20 C-ter fragment DAMMIF model
Synchrotron SAXS data from solutions of A20 C-ter fragment in 25 mM Tris-HCl, 300 mM NaCl, pH 7.5 were collected using size exclusion chromatography coupled SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.3 m and at a wavelength of λ = 0.0931 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Data were collected at at 20°C during the course of elution (3000 successive 1 second frames). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the resulting subtracted curves were scaled and averaged.

Concentration min = UNKNOWN. Concentration max = UNKNOWN

DNA polymerase processivity factor component A20 C-ter fragment (A20 C-ter)
Mol. type   Protein
Organism   Vaccinia virus
Olig. state   Monomer
Mon. MW   16.7 kDa
 
UniProt   P20995 (304-426)
Sequence   FASTA