Conformational States of Exchange Protein Directly Activated by cAMP (EPAC1) Revealed by Ensemble Modeling and Integrative Structural Biology.

White MA, Tsalkova T, Mei FC, Cheng X, Cells 9(1) (2019) Europe PMC

SASDCQ6 – Rap guanine nucleotide exchange factor 3 (isoform3) - apo form

Rap guanine nucleotide exchange factor 3
MWI(0) 73 kDa
MWexpected 100 kDa
VPorod 180 nm3
log I(s) 2.16×10-1 2.16×10-2 2.16×10-3 2.16×10-4
Rap guanine nucleotide exchange factor 3 small angle scattering data  s, nm-1
ln I(s)
Rap guanine nucleotide exchange factor 3 Guinier plot ln 2.17×10-1 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Rap guanine nucleotide exchange factor 3 Kratky plot 1.104 0 √3 sRg
p(r)
Rap guanine nucleotide exchange factor 3 pair distance distribution function Rg: 3.4 nm 0 Dmax: 11 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 SWISSMODEL model
log I(s)
 s, nm-1
Rap guanine nucleotide exchange factor 3 DAMMIF model
SAXS data from solutions of Rap guanine nucleotide exchange factor 3 (isoform3) in 1mM EDTA, 10mM DTT, 500mM NaCl, 10mM Tris, pH 9 were collected using a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) equipped with a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (I(s) vs s, where s = 4πsin θ/λ and 2θ is the scattering angle). A sample concentration of 0.5 mg/ml was measured at 10°C. SAXS data were acquired from 12 successive 21600 second frames. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted to generate the data displayed for this entry.

Rap guanine nucleotide exchange factor 3 (EPAC1B)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   100.0 kDa
 
UniProt   O95398-3 (2-881)
Sequence   FASTA
 
PDB ID   4F7Z