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Synchrotron SAXS data from solutions of TET12(1.10)SN-c6 in 20 mM Tris 150 mM NaCl 10% glycerol, pH 7.5 were collected on the P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 1M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.9 and 3.6 mg/ml were measured at 20°C. 20 successive 0.045 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the different curves were scaled for protein concentration.
To detect any potential concentration dependent effects a series consisting of 3 concentrations was measured. The highest concentration was 3.6 mg/ml, while the lowest concentrations was 0.9 mg/ml. For each sample data was collected over 20 frames lasting 0.05 s. Frames were carefully inspected for radiation damage and those not showing any radiation damage were then averaged. Before and after each sample scattering, matching buffer was measured and subtracted from sample scattering. The protein did not exhibit any concentration dependent effects, therefore the curve measured for the sample with the highest concentration was used for subsequent analysis. Scattering curves were analyzed using PRIMUS software.
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TET12(1.10)SN-c6
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| Mol. type |
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Protein |
| Organism |
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synthetic construct |
| Olig. state |
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Monomer |
| Mon. MW |
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54.3 kDa |
| Sequence |
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FASTA |
| |
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s, nm-1
