| MWexperimental | 101 | kDa |
| MWexpected | 82 | kDa |
| VPorod | 155 | nm3 |
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log I(s)
6.72×10-3
6.72×10-4
6.72×10-5
6.72×10-6
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s, nm-1
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The structure of SALM5 suggests a dimeric assembly for the presynaptic RPTP ligand recognition.
Karki S, Paudel P, Sele C, Shkumatov AV, Kajander T, Protein Eng Des Sel (2018) Europe PMCSASDD23 – Leucine-rich repeat and fibronectin type-III domain-containing protein 5: Fragment SALM5 LRR-Ig
Leucine-rich repeat and fibronectin type-III domain-containing protein 5|
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Fits and models
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Rg, nm
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Synchrotron SAXS data from solutions of Leucine-rich repeat and fibronectin type-III domain-containing protein 5 (fragment SALM5 (LRR-Ig)) in 30 mM Tris-Cl, 150 mM NaCl, 3% v/v glycerol, pH 7.5, were collected on the B21 beam line at the Diamond Light Source storage ring (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4.0 m and at a wavelength of λ = 0.1005 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 5 mg/ml were measured at 15°C. 18 successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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