MbsTA

Diana Freire.

SASDD43 – Mycobacterial suicide toxin (MbsT)

Mycobacterial cidal toxin
MWI(0) 18 kDa
MWexpected 20 kDa
VPorod 30 nm3
log I(s) 1.86×104 1.86×103 1.86×102 1.86×101
Mycobacterial cidal toxin small angle scattering data  s, nm-1
ln I(s)
Mycobacterial cidal toxin Guinier plot ln 1.86×104 Rg: 1.8 nm 0 (1.8 nm)-2 s2
(sRg)2I(s)/I(0)
Mycobacterial cidal toxin Kratky plot 1.104 0 √3 sRg
p(r)
Mycobacterial cidal toxin pair distance distribution function Rg: 1.7 nm 0 Dmax: 5.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Mycobacterial cidal toxin SREFLEX model
log I(s)
 s, nm-1
Mycobacterial cidal toxin PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of Mycobacterial suicide toxin (MbsT) in 30 mM Tris-HCl, 200 mM NaCl, 10% glycerol, pH 7.5, were collected on the P12 beam line at the PETRA III storage ring (Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 2 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). Solute concentrations ranging between 0.4 and 7.4 mg/ml were measured at 20.3°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Mycobacterial cidal toxin (MbcT)
Mol. type   Protein
Organism   Mycobacterium tuberculosis H37Rv
Olig. state   Monomer
Mon. MW   20.4 kDa
 
UniProt   P9WLP9 (1-186)
Sequence   FASTA
 
PDB ID   6FKG