Molecular Basis for Membrane Recruitment by the PX and C2 Domains of Class II Phosphoinositide 3-Kinase-C2α.

Chen KE, Tillu VA, Chandra M, Collins BM, Structure (2018) Europe PMC

SASDD66 – Phox homologue (PX) - C2 domains of human phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α)

Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
MWexperimental 35 kDa
MWexpected 33 kDa
VPorod 43 nm3
log I(s) 2.38×10-2 2.38×10-3 2.38×10-4 2.38×10-5
Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha small angle scattering data  s, nm-1
ln I(s)
Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha Guinier plot ln 2.38×10-2 Rg: 2.6 nm 0 (2.6 nm)-2 s2
(sRg)2I(s)/I(0)
Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha Kratky plot 1.104 0 √3 sRg
p(r)
Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha pair distance distribution function Rg: 2.7 nm 0 Dmax: 9.3 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha DAMFILT model
Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha DAMMIN model
log I(s)
 s, nm-1
Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha CORAL model
Synchrotron SAXS data from solutions of the C2 domains of human PI3KC2α (Phox homologue (PX)) in 25 mM Tris 200 mM NaCl 5% v/v glycerol 0.5 mM TCEP, pH 8.5 were collected on the SAXS/WAXS camera at the Australian Synchrotron storage ring (Melbourne, Australia) using a Pilatus 1M detector at a wavelength of λ = 0.10332 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size exclusion chromatography SAXS (SEC-SAXS) was used. 14 successive 1 second frames were collected through the SEC elution peak of the protein. The data were normalized to the intensity of the transmitted beam and radially averaged. The resulting 1D data frames were then scaled and then averaged. The scattering of an appropriate solvent-blank from the SEC column was subtracted.

SEC-SAXS details: Column type: S200 5/150 GL column; Flow rate: 0.45 ml/min; Sample temperature, 10°C; Sample injection concentration: 10.5 mg/ml. The ab initio models represent the spatially aligned and volume occupancy corrected (averaged) representation of the protein obtained from 20 individual reconstructions (top, DAMFILT model: NSD = 0.66 +/- 0.02; resolution estimate = 3.3 nm) and the best-fit individual DAMMIN reconstruction (bottom) displayed with the corresponding individual model fit to the SAXS data.

Phox Homology (PX) - C2 domains of human Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (PI3KC2α)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   32.7 kDa
 
UniProt   O00443 (1405-1686)
Sequence   FASTA