| MWexperimental | 41 | kDa |
| MWexpected | 41 | kDa |
| VPorod | 70 | nm3 |
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log I(s)
1.40×10-1
1.40×10-2
1.40×10-3
1.40×10-4
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s, nm-1
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Structural Insight into Catalysis by the Flavin-Dependent NADH Oxidase (Pden_5119) of Paracoccus denitrificans.
Kryl M, Sedláček V, Kučera I, Int J Mol Sci 24(4) (2023) Europe PMCSASDD73 – Flavin reductase Pden_5119
NADPH-dependent FMN reductase|
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Fits and models
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SAXS data from solutions of flavin reductase Pden_5119 in 50 mM sodium phosphate buffer, 300 mM NaCl, 500 mM imidazole, pH 8, were collected on a Rigaku BioSAXS-1000 instrument at the Central European Institute of Technology, CEITEC, (Brno, Czech Republic) using a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.5 and 7.5 mg/ml were measured at 4°C. 30 successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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