Structural analysis of human NHLRC2, mutations of which are associated with FINCA disease.

Biterova E, Ignatyev A, Uusimaa J, Hinttala R, Ruddock LW, PLoS One 13(8):e0202391 (2018) Europe PMC

SASDD86 – Full-length human NHLRC2 ( NHL repeat-containing protein 2 )

NHL repeat-containing protein 2
MWexperimental 71 kDa
MWexpected 80 kDa
VPorod 121 nm3
log I(s) 3.00×10-1 3.00×10-2 3.00×10-3 3.00×10-4
NHL repeat-containing protein 2 small angle scattering data  s, nm-1
ln I(s)
NHL repeat-containing protein 2 Guinier plot ln 3.01×10-1 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
NHL repeat-containing protein 2 Kratky plot 1.104 0 √3 sRg
p(r)
NHL repeat-containing protein 2 pair distance distribution function Rg: 3.8 nm 0 Dmax: 14.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
NHL repeat-containing protein 2 DAMFILT model
Synchrotron SAXS data from solutions of full-length human NHLRC2 (NHL repeat-containing protein 2 ) in 20 mM BisTris, 100 mM NaCl, 2 mM DTT, pH 6.5, were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 10°C. 60 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

NHL repeat-containing protein 2 (NHLRC2)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   80.4 kDa
 
UniProt   Q8NBF2 (1-726)
Sequence   FASTA