Structural Plasticity of Neurexin 1α: Implications for its Role as Synaptic Organizer.

Liu J, Misra A, Reddy MVVVS, White MA, Ren G, Rudenko G, J Mol Biol 430(21):4325-4343 (2018) Europe PMC

SASDDA5 – Neurexin 1a L5L6 with ss6 insert

Neurexin 1a L5L6 with ss6 insert
MWI(0) 45 kDa
MWexpected 45 kDa
VPorod 70 nm3
log I(s) 4.51×104 4.51×103 4.51×102 4.51×101
Neurexin 1a L5L6 with ss6 insert small angle scattering data  s, nm-1
ln I(s)
Neurexin 1a L5L6 with ss6 insert Guinier plot ln 4.51×104 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Neurexin 1a L5L6 with ss6 insert Kratky plot 1.104 0 √3 sRg
p(r)
Neurexin 1a L5L6 with ss6 insert pair distance distribution function Rg: 3.3 nm 0 Dmax: 12.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Neurexin 1a L5L6 with ss6 insert Rg histogram Rg, nm
log I(s)
 s, nm-1
Neurexin 1a L5L6 with ss6 insert CORAL model
SAXS data from solutions of Neurexin 1a L5L6 with ss6 insert in 20 mM HEPES pH 8, 150 mM NaCl, 0.5mM CaCl2, pH 8 were collected on a Rigaku BioSAXS-1000 instrument at the Sealy Center For Structural Biology, UTMB-G (Galveston, TX, USA) using a Kratky-2D Rigaku BioSAXS-1000 detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.15418 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2 and 4 mg/ml were measured at 10°C. Three successive 10 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Neurexin 1a L5L6 with ss6 insert (L5L6ss6)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   45.5 kDa
 
UniProt   Q9ULB1 (904-1307)
Sequence   FASTA