| MWI(0) | 36 | kDa |
| MWexpected | 28 | kDa |
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log I(s)
3.91×10-2
3.91×10-3
3.91×10-4
3.91×10-5
|
s, nm-1
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Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa.
Fulara A, Vandenberghe I, Read RJ, Devreese B, Savvides SN, Sci Rep 8(1):16760 (2018) Europe PMCSASDDB8 – Periplasmic domain of inner membrane protein GspL, dimer
Type II secretion system protein L, periplasmic domain|
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Fits and models
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Synchrotron SAXS
data from solutions of
Periplasmic domain of inner membrane protein GspL, dimer
in
50 mM TRIS, 100 mM NaCl, pH 7.5
were collected
on the
SWING beam line
at the SOLEIL storage ring
(Saint-Aubin, France)
using a AVIEX PCCD170170 detector
at a wavelength of λ = 0.1033 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A sample
at 13.7 mg/ml was injected
onto a column
at 15°C.
19 successive
frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Sample detector distance = UNKNOWN. X-ray Exposure time = UNKNOWN. SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN |
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