Synthetic FRET biosensor Twitch 2B

Pablo Trigo Mourino.

SASDDC7 – Synthetic FRET biosensor Twitch 2B N532F Ca-free

Synthetic FRET biosensor Twitch2B N532F
MWexperimental 55 kDa
MWexpected 62 kDa
log I(s) 3.65×101 3.65×100 3.65×10-1 3.65×10-2
Synthetic FRET biosensor Twitch2B N532F small angle scattering data  s, nm-1
ln I(s)
Synthetic FRET biosensor Twitch2B N532F Guinier plot ln 3.66×101 Rg: 3.9 nm 0 (3.9 nm)-2 s2
(sRg)2I(s)/I(0)
Synthetic FRET biosensor Twitch2B N532F Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of the synthetic FRET biosensor, Twitch 2B N532F, in the absence of calcium, in 20 mM MOPS 100 mM NaCl 10 mM EDTA, pH 7 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.0995 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 20°C. 12 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Synthetic FRET biosensor Twitch2B N532F (TW6)
Mol. type   Protein
Organism   synthetic construct
Olig. state   Monomer
Mon. MW   62.4 kDa
Sequence   FASTA