Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2) in Complex with 40 bp DNA

Marian Vogt.

SASDDG3 – Solution Structure of Archaeal Biofilm Regulator 2 (AbfR2) in Complex with DNA

Sa0446 binding sequence 40bp
Transcriptional regulator Lrs14-like protein
MWI(0) 68 kDa
MWexpected 57 kDa
VPorod 60 nm3
log I(s) 6.77×101 6.77×100 6.77×10-1 6.77×10-2
Sa0446 binding sequence 40bp Transcriptional regulator Lrs14-like protein small angle scattering data  s, nm-1
ln I(s)
Sa0446 binding sequence 40bp Transcriptional regulator Lrs14-like protein Guinier plot ln 6.77×101 Rg: 3.4 nm 0 (3.4 nm)-2 s2
(sRg)2I(s)/I(0)
Sa0446 binding sequence 40bp Transcriptional regulator Lrs14-like protein Kratky plot 1.104 0 √3 sRg
p(r)
Sa0446 binding sequence 40bp Transcriptional regulator Lrs14-like protein pair distance distribution function Rg: 3.6 nm 0 Dmax: 12.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Sa0446 binding sequence 40bp Transcriptional regulator Lrs14-like protein DAMMIF model
log I(s)
 s, nm-1
Sa0446 binding sequence 40bp Transcriptional regulator Lrs14-like protein DAMMIF model
Synchrotron SAXS data from solutions of Archaeal biofilm regulator 2 (AbfR2) in complex with 40 base-pair dsDNA (Sa0446 binding sequence) in 300 mM NaCl, 20 mM HEPES, pH 7.5, were collected on the BM29 beam line at the ESRF storage ring (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.63 mg/ml was measured at 4°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN. Concentration min = UNKNOWN

Sa0446 binding sequence 40bp (40bp dsDNA)
Mol. type   DNA
Olig. state   Monomer
Mon. MW   24.7 kDa
Sequence   FASTA
 
Transcriptional regulator Lrs14-like protein (AbfR2)
Mol. type   Protein
Organism   Sulfolobus acidocaldarius
Olig. state   Dimer
Mon. MW   16.4 kDa
 
UniProt   Q4J9G1 (1-123)
Sequence   FASTA