| MWI(0) | 45 | kDa |
| MWexpected | 51 | kDa |
| VPorod | 86 | nm3 |
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log I(s)
4.25×101
4.25×100
4.25×10-1
4.25×10-2
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s, nm-1
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Biosynthesis of mycobacterial methylmannose polysaccharides requires a unique 1-O-methyltransferase specific for 3-O-methylated mannosides.
Ripoll-Rozada J, Costa M, Manso JA, Maranha A, Miranda V, Sequeira A, Ventura MR, Macedo-Ribeiro S, Pereira PJB, Empadinhas N, Proc Natl Acad Sci U S A 116(3):835-844 (2019) Europe PMCSASDDJ6 – SAM-dependent O-Methyltransferase from Mycobacterium hassiacum
Methyltransferase domain protein|
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Fits and models
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Synchrotron SAXS data from solutions of Mycobacterium hassiacum Methyltransferase in 20 mM bis-tris propane (pH 7.5), 50 mM NaCl, 5% glycerol and 2 mM DTT were collected on the BM29 BIOSAXS beam line at the ESRF synchrotron (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.867 m and at a wavelength of λ = 0.0992 nm , the range of momentum transfer 0.036 < s < 4.939 nm-1 was covered (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.6 and 12.9 mg/ml were measured at 10°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentrations were extrapolated to infinite dilution.
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