Synchrotron SAXS data from solutions of calcium-loaded calbindin-D28K in 20 mM Tris, 150 mM NaCl, 3 mM CaCl2, pH 7.8, were collected on the B21 beam line at the Diamond Light Source (Oxfordshire, UK) using a Pilatus 2M detector at a sample-detector distance of 4 m and at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 11.50 mg/ml was measured at 25°C. 15 successive 3 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN
|
Calbindin
(CALB1)
|
Mol. type |
|
Protein |
Organism |
|
Homo sapiens |
Olig. state |
|
Monomer |
Mon. MW |
|
30.3 kDa |
|
UniProt |
|
P05937
|
Sequence |
|
FASTA |
|
PDB ID
|
|
6FIE
|
|
PDB ID
|
|
2G9B
|
|
|