Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein.

Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C, PLoS One 13(1):e0191162 (2018) Europe PMC

SASDDL7 – anti-DISC1 single-domain camelid antibody VHH B5

anti-DISC1 single-domain camelid antibody VHH B5
MWI(0) 15 kDa
MWexpected 14 kDa
VPorod 21 nm3
log I(s) 1.51×101 1.51×100 1.51×10-1 1.51×10-2
anti-DISC1 single-domain camelid antibody VHH B5 small angle scattering data  s, nm-1
ln I(s)
anti-DISC1 single-domain camelid antibody VHH B5 Guinier plot ln 1.52×101 Rg: 1.8 nm 0 (1.8 nm)-2 s2
(sRg)2I(s)/I(0)
anti-DISC1 single-domain camelid antibody VHH B5 Kratky plot 1.104 0 √3 sRg
p(r)
anti-DISC1 single-domain camelid antibody VHH B5 pair distance distribution function Rg: 1.9 nm 0 Dmax: 8.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
anti-DISC1 single-domain camelid antibody VHH B5 GASBOR model
anti-DISC1 single-domain camelid antibody VHH B5 GASBOR model
log I(s)
 s, nm-1
anti-DISC1 single-domain camelid antibody VHH B5 DAMMIF model
anti-DISC1 single-domain camelid antibody VHH B5 DAMMIF model
Synchrotron SAXS data from solutions of the anti-DISC1 single-domain camelid antibody VHH B5 in 25 mM Tris, 150 mM NaCl, pH 7.4 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a wavelength of λ = 0.1 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 2.8 and 5.6 mg/ml were measured at 20°C. 10 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

The data was obtained from the merged SAXS profiles measured from two samples at 2.8 and 5.6 mg/mL.

anti-DISC1 single-domain camelid antibody VHH B5 (VHH B5)
Mol. type   Protein
Olig. state   Monomer
Mon. MW   13.9 kDa
Sequence   FASTA