Redox Modulation of Oligomeric State in Proline Utilization A.

Korasick DA, Campbell AC, Christgen SL, Chakravarthy S, White TA, Becker DF, Tanner JJ, Biophys J 114(12):2833-2843 (2018) Europe PMC

SASDDQ3 – Proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) collected by SEC-SAXS

Bifunctional protein PutA

MW^experimental	434	kDa
MW^expected	430	kDa
V^Porod	582	nm³

log I(s) 4.61×10² 4.61×10¹ 4.61×10⁰ 4.61×10^-1

Bifunctional protein PutA small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 4.61×10² R_g: 5.2 nm 0 (5.2 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 5.2 nm 0 D_max: 14.2 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.3

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.234
0.701

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Bifunctional protein PutA PDB (PROTEIN DATA BANK) model

Synchrotron SAXS data from solutions of proline utilization A from Bradyrhizobium diazoefficiens (formerly Bradyrhizobium japonicum) were collected using SEC-SAXS in 50 mM Tris, 50 mM NaCl, 0.5 mM TCEP, 5% (v/v) glycerol, pH 7.8 on the BioCAT 18ID beam line at the Advanced Photon Source (APS, Argonne, IL, USA) using a Pilatus 100K detector at a sample-detector distance of 3.5 m and at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). The SEC-SAXS elution was collected at 22°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. X-ray Exposure time = UNKNOWN. Number of frames = UNKNOWN. SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN

Bifunctional protein PutA (BjPutA)
Mol. type		Protein
Organism		Bradyrhizobium diazoefficiens
Olig. state		Tetramer
Mon. MW		107.6 kDa

UniProt		Q89E26
Sequence		FASTA

PDB ID		3HAZ