Crystal Structure of Aldehyde Dehydrogenase 16 Reveals Trans-Hierarchical Structural Similarity and a New Dimer.

Liu LK, Tanner JJ, J Mol Biol (2018) Europe PMC

SASDE47 – Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16): 2 mg/ml

Aldehyde dehydrogenase 16 from Loktanella sp.
MWexperimental 142 kDa
MWexpected 161 kDa
VPorod 202 nm3
log I(s) 2.67×102 2.67×101 2.67×100 2.67×10-1
Aldehyde dehydrogenase 16 from Loktanella sp. small angle scattering data  s, nm-1
ln I(s)
Aldehyde dehydrogenase 16 from Loktanella sp. Guinier plot ln 2.68×102 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Aldehyde dehydrogenase 16 from Loktanella sp. Kratky plot 1.104 0 √3 sRg
p(r)
Aldehyde dehydrogenase 16 from Loktanella sp. pair distance distribution function Rg: 3.6 nm 0 Dmax: 10.9 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Aldehyde dehydrogenase 16 from Loktanella sp. PYMOL model
Synchrotron SAXS data from solutions of aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16) in 20 mM Tris-HCl, 100 mM NaCl, 2.0% glycerol, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. 32 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16)
Mol. type   Protein
Organism   Loktanella sp. 3ANDIMAR09
Olig. state   Dimer
Mon. MW   80.7 kDa
 
UniProt   A0A0Q3EUQ3 (1-766)
Sequence   FASTA