SASDE67 – Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16): 6 mg/ml

Aldehyde dehydrogenase 16 from Loktanella sp.

MW^experimental	149	kDa
MW^expected	161	kDa
V^Porod	207	nm³

log I(s) 1.09×10³ 1.09×10² 1.09×10¹ 1.09×10⁰

Aldehyde dehydrogenase 16 from Loktanella sp. small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

Aldehyde dehydrogenase 16 from Loktanella sp. Guinier plot

ln 1.10×10³ R_g: 3.5 nm 0 (3.5 nm)^-2 s²

(sR_g)²I(s)/I(0)

Aldehyde dehydrogenase 16 from Loktanella sp. Kratky plot

1.104 0 √3 sR_g

p(r)	R_g: 3.5 nm 0 D_max: 10.6 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.6

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.122
0.423

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Aldehyde dehydrogenase 16 from Loktanella sp. PYMOL model

Synchrotron SAXS data from solutions of aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16) in 20 mM Tris-HCl, 100 mM NaCl, 2.0% glycerol, 0.5 mM Tris(3-hydroxypropyl)phosphine, pH 8 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS; Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.127 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 6.00 mg/ml was measured at 10°C. 32 successive 0.300 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN

Aldehyde dehydrogenase 16 from Loktanella sp. (LsALDH16)
Mol. type		Protein
Organism		Loktanella sp. 3ANDIMAR09
Olig. state		Dimer
Mon. MW		80.7 kDa

UniProt		A0A0Q3EUQ3 (1-766)
Sequence		FASTA