Farnesylation of human guanylate binding protein 1 as safety mechanism preventing structural rearrangements and uninduced dimerization.

Lorenz C, Ince S, Zhang T, Cousin A, Batra-Safferling R, Nagel-Steger L, Herrmann C, Stadler AM, FEBS J (2019) Europe PMC

SASDEG8 – Human Guanylate Binding Protein 1 with GppNHp (hGBP1 + GppNHp), dimer from SEC-SAXS

human Guanylate-binding protein 1
MWexperimental 159 kDa
MWexpected 138 kDa
VPorod 270 nm3
log I(s) 1.55×102 1.55×101 1.55×100 1.55×10-1
human Guanylate-binding protein 1 small angle scattering data  s, nm-1
ln I(s)
human Guanylate-binding protein 1 Guinier plot ln 1.56×102 Rg: 5.5 nm 0 (5.5 nm)-2 s2
(sRg)2I(s)/I(0)
human Guanylate-binding protein 1 Kratky plot 1.104 0 √3 sRg
p(r)
human Guanylate-binding protein 1 pair distance distribution function Rg: 5.7 nm 0 Dmax: 22 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human Guanylate Binding Protein 1 with GppNHp (hGBP1 + GppNHp), dimer from SEC-SAXS Rg histogram Rg, nm
log I(s)
 s, nm-1
human Guanylate-binding protein 1 PYMOL model
log I(s)
 s, nm-1
human Guanylate-binding protein 1 PYMOL model
Synchrotron SAXS data from solutions of Human Guanylate Binding Protein 1 with guanylyl imidodiphosphate (hGBP1 + GppNHp) in 50 mM Tris-HCl, 5 mM MgCl2, 150 mM NaCl, 0.2 mM GppNHp pH 7.9 were collected using size-exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed at 20°C using the following parameters: Column: UNKNOWN; Flow rate: UNKNOWN mL/min; Total acquisition time: 3600 s; Sample injection concentration: 55 mg/mL; Injection volume: UNKNOWN μL. The data obtained through the sample elution peak (collected as successive 1 s frames) were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.

human Guanylate-binding protein 1 (hGBP1)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   69.2 kDa
 
UniProt   P32455 (1-592)
Sequence   FASTA