Nitrosative stress sensing in Porphyromonas gingivalis: structure of and heme binding by the transcriptional regulator HcpR.

Belvin BR, Musayev FN, Burgner J, Scarsdale JN, Escalante CR, Lewis JP, Acta Crystallogr D Struct Biol 75(Pt 4):437-450 (2019) Europe PMC

SASDEN9 – HcpR transcriptional regulator of Porphyromonas gingivalis

Hcp Transcriptional regulator
MWexperimental 44 kDa
MWexpected 51 kDa
VPorod 76 nm3
log I(s) 2.82×102 2.82×101 2.82×100 2.82×10-1
Hcp Transcriptional regulator small angle scattering data  s, nm-1
ln I(s)
Hcp Transcriptional regulator Guinier plot ln 2.83×102 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Hcp Transcriptional regulator Kratky plot 1.104 0 √3 sRg
p(r)
Hcp Transcriptional regulator pair distance distribution function Rg: 2.9 nm 0 Dmax: 10.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
HcpR transcriptional regulator of Porphyromonas gingivalis Rg histogram Rg, nm
Hcp Transcriptional regulator EOM/RANCH model
Hcp Transcriptional regulator EOM/RANCH model
Hcp Transcriptional regulator EOM/RANCH model
Synchrotron SAXS data from solutions of Porphyromonas gingivalis HcpR in 25 mM Trish 150 mM NaCl, 2mM TCEP pH 7.5 were collected on the SIBYLS beam line at the Lawrence Berkeley National Laboratory (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 3.5 meters at a wavelength of λ = 0.103 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.0 mg/ml was measured at 21°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Number of frames = UNKNOWN

Hcp Transcriptional regulator (HcpR)
Mol. type   Protein
Organism   Porphyromonas gingivalis
Olig. state   Dimer
Mon. MW   25.5 kDa
 
UniProt   Q7MVK4 (1-227)
Sequence   FASTA