SASDEU9 – Legionella pneumophila Phosphocholinase AnkX

Phosphocholinase AnkX

MW^experimental	109	kDa
MW^expected	107	kDa
V^Porod	145	nm³

log I(s) 8.63×10¹ 8.63×10⁰ 8.63×10^-1 8.63×10^-2

Phosphocholinase AnkX small angle scattering data

s, nm^-1

Log plot Log-Log plot

ln I(s)

ln 8.63×10¹ R_g: 4.3 nm 0 (4.3 nm)^-2 s²

(sR_g)²I(s)/I(0)

1.104 0 √3 sR_g

p(r)	R_g: 4.3 nm 0 D_max: 14.7 nm

Data validation

Experimental data range

p(r) fit to data

Metric

Value

s_min D_max / π

0.9

N_Shannon

Worse

Better

Metric

Value

p_cormap

χ²

0.046
0.728

Worse

Better

Fits and models

log I(s)	s, nm^-1
+3 Δ/σ -3	s, nm^-1

Synchrotron SAXS data from solutions of phosphocholinase AnkX in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the BM29 camera at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a wavelength of λ = 0.09919 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 8.00 mg/ml was measured. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

X-ray exposure time = UNKNOWN. Sample temperature = UNKNOWN.

Phosphocholinase AnkX (AnkX)
Mol. type		Protein
Organism		Legionella pneumophila
Olig. state		Monomer
Mon. MW		107.2 kDa

UniProt		Q5ZXN6
Sequence		FASTA