Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.

Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG, Structure (2019) Europe PMC

SASDEW9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO

Cytohesin-2 ARF nucleotide-binding site opener
MWexperimental 94 kDa
MWexpected 93 kDa
VPorod 145 nm3
log I(s) 4.39×101 4.39×100 4.39×10-1 4.39×10-2
Cytohesin-2 ARF nucleotide-binding site opener small angle scattering data  s, nm-1
ln I(s)
Cytohesin-2 ARF nucleotide-binding site opener Guinier plot ln 4.40×101 Rg: 4.8 nm 0 (4.8 nm)-2 s2
(sRg)2I(s)/I(0)
Cytohesin-2 ARF nucleotide-binding site opener Kratky plot 1.104 0 √3 sRg
p(r)
Cytohesin-2 ARF nucleotide-binding site opener pair distance distribution function Rg: 5.1 nm 0 Dmax: 19.7 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Cytohesin-2 ARF nucleotide-binding site opener GASBOR model
Synchrotron SAXS data from solutions of the cytohesin-2; ARF nucleotide-binding site opener, ARNO, in 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Dectris Pilatus 1M detector at a wavelength of λ = 0.993 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 10.00 mg/ml was measured at 15°C. 1500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Sample detector distance = UNKNOWN

Cytohesin-2 ARF nucleotide-binding site opener (ARNO)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Dimer
Mon. MW   46.6 kDa
 
UniProt   Q99418 (1-400)
Sequence   FASTA