| MWexperimental | 192 | kDa |
| MWexpected | 184 | kDa |
| VPorod | 259 | nm3 |
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log I(s)
4.99×101
4.99×100
4.99×10-1
4.99×10-2
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s, nm-1
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Structural Characterisation of the Arabidopsis thaliana APC4
Steven De Gieter.SASDEY6 – Arabidopsis thaliana anaphase promoting complex/cyclosome subunit 4 (APC4-strep)
Anaphase Promoting Complex/Cyclosome Subunit 4|
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Fits and models
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Synchrotron SAXS data from solutions of anaphase promoting complex/cyclosome subunit 4 in 10 mM Tris ,150 mM NaCl, 1 mM TCEP, pH 8 were collected using size exclusion chromatography SAXS (SEC-SAXS) on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (l(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). SEC-SAXS was performed using the following parameters: Column type: Shodex KW404-4F; Injection volume: 50 µl; Sample injection concentration: 8.1 mg/ml. 69 successive 1 second frames were collected through the elution peak (measured at 20°C). The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted and the individual subtracted data sets were scaled and averaged to generate the scattering profile displayed in this entry.
SEC column = UNKNOWN. Sample injection volume = UNKNOWN. Flow rate = UNKNOWN |
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