Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.

Yadav DK, Zigáčková D, Zlobina M, Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ, Nucleic Acids Res (2019) Europe PMC

SASDF49 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long

Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4
ADP-ribosylation factor1 - long
MWexperimental 34 kDa
MWexpected 36 kDa
VPorod 53 nm3
log I(s) 1.02×10-2 1.02×10-3 1.02×10-4 1.02×10-5
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 ADP-ribosylation factor1 - long small angle scattering data  s, nm-1
ln I(s)
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 ADP-ribosylation factor1 - long Guinier plot ln 1.02×10-2 Rg: 2.5 nm 0 (2.5 nm)-2 s2
(sRg)2I(s)/I(0)
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 ADP-ribosylation factor1 - long Kratky plot 1.104 0 √3 sRg
p(r)
Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 ADP-ribosylation factor1 - long pair distance distribution function Rg: 2.5 nm 0 Dmax: 7.0 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long in 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol, pH 6.8 were collected on the B21 beam line at the Diamond Light Source (Didcot, UK) using a Pilatus 2M detector at a wavelength of λ = 0.1025 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 5.00 mg/ml was measured at 15°C. One 3 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Storage temperature = UNKNOWN. Sample detector distance = UNKNOWN

Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 (STAU1-RBD34)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   19.7 kDa
 
UniProt   O95793 (183-355)
Sequence   FASTA
 
ADP-ribosylation factor1 - long (ARF1 RNA - long)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   16.1 kDa
Sequence   FASTA