Quaternary structure of α-amino-β-carboxymuconate-ϵ-semialdehyde decarboxylase (ACMSD) controls its activity.

Yang Y, Davis I, Matsui T, Rubalcava I, Liu A, J Biol Chem 294(30):11609-11621 (2019) Europe PMC

SASDFM5 – Mutant 2-amino-3-carboxymuconate 6-semialdehyde decarboxylase, H110A tetramer, at pH 8.5

2-amino-3-carboxymuconate 6-semialdehyde decarboxylase
MWexperimental 159 kDa
MWexpected 159 kDa
VPorod 238 nm3
log I(s) 1.33×101 1.33×100 1.33×10-1 1.33×10-2
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase small angle scattering data  s, nm-1
ln I(s)
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase Guinier plot ln 1.33×101 Rg: 5.2 nm 0 (5.2 nm)-2 s2
(sRg)2I(s)/I(0)
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase Kratky plot 1.104 0 √3 sRg
p(r)
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase pair distance distribution function Rg: 5.6 nm 0 Dmax: 19 nm

Data validation

Fits and models

log I(s)
 s, nm-1
2-amino-3-carboxymuconate 6-semialdehyde decarboxylase CORAL model
Synchrotron SAXS data from solutions of mutant ACMSD (H110A) in 50 mM Tris, 5 mM DTT, pH 8.5 were collected on the BL4-2 beam line at the Stanford Synchrotron Radiation Lightsource (SSRL; Menlo Park, CA, USA) using a Pilatus3 X 1M detector at a sample-detector distance of 2.5 m and at a wavelength of λ = 0.1228 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 0.05 ml sample at mg/ml was injected onto a GE Superdex 200 Increase 5/150 column at 25°C. 500 successive 1 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Flow rate = UNKNOWN

2-amino-3-carboxymuconate 6-semialdehyde decarboxylase (ACMSD)
Mol. type   Protein
Organism   Pseudomonas fluorescens
Olig. state   Tetramer
Mon. MW   39.7 kDa
 
UniProt   Q83V25 (1-334)
Sequence   FASTA