| MWexperimental | 300 | kDa |
| MWexpected | 183 | kDa |
| VPorod | 352 | nm3 |
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log I(s)
7.18×101
7.18×100
7.18×10-1
7.18×10-2
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s, nm-1
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Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling.
Remesh SG, Verma SC, Chen JH, Ekman AA, Larabell CA, Adhya S, Hammel M, Nat Commun 11(1):2905 (2020) Europe PMCSASDFV5 – DNA-binding protein HU-alpha bound to 80 base-pair DNA at pH 6.5 with 150 mM NaCl
80bp_DNA Forward80bp_DNA Reverse
DNA-binding protein HU-alpha
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Fits and models
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Synchrotron SAXS data from solutions of DNA-binding protein HU-alpha bound to 80 base-pair DNA in 10 mM Bis-Tris, 150 mM NaCl, pH 6.5 were collected on the 12.3.1 (SIBYLS) beam line at the Advanced Light Source (ALS) storage ring (Berkeley, CA, USA) using a Pilatus3 X 2M detector at a sample-detector distance of 1.5 m and at a wavelength of λ = 0.103 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). 300 successive 3 second frames were collected at 10°C. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
Storage temperature = UNKNOWN. Concentration = UNKNOWN |
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