Structure of pre-miR-31 reveals an active role in Dicer-TRBP complex processing.

Ma S, Kotar A, Hall I, Grote S, Rouskin S, Keane SC, Proc Natl Acad Sci U S A 120(39):e2300527120 (2023) Europe PMC

SASDRF9 – Precursor microRNA 31

Precursor microRNA 31
MWexperimental 26 kDa
MWexpected 23 kDa
VPorod 31 nm3
log I(s) 1.21×10-2 1.21×10-3 1.21×10-4 1.21×10-5
Precursor microRNA 31 small angle scattering data  s, nm-1
ln I(s)
Precursor microRNA 31 Guinier plot ln 1.21×10-2 Rg: 2.9 nm 0 (2.9 nm)-2 s2
(sRg)2I(s)/I(0)
Precursor microRNA 31 Kratky plot 1.104 0 √3 sRg
p(r)
Precursor microRNA 31 pair distance distribution function Rg: 3.1 nm 0 Dmax: 10.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Precursor microRNA 31 PDB (PROTEIN DATA BANK) model
Synchrotron SAXS data from solutions of precursor microRNA 31 in 50 mM potassium phosphate, 50 mM NaCl, 1 mM MgCl2, pH 7.5 were collected on the BioCAT 18ID beam line at the Advanced Photon Source (APS), Argonne National Laboratory (Lemont, IL, USA) using a Pilatus 100K detector at a sample-detector distance of 3.7 m and at a wavelength of λ = 0.1033 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). In-line size-exclusion chromatography (SEC) SAS was employed. The SEC parameters were as follows: A 225.00 μl sample at 2.3 mg/ml was injected at a 0.60 ml/min flow rate onto a GE Superdex 75 Increase 10/300 column at 20°C. 2248 successive 0.200 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Precursor microRNA 31 (pre-miR-31)
Mol. type   RNA
Organism   Homo sapiens
Olig. state   Monomer
Mon. MW   22.9 kDa
Sequence   FASTA
 
PDB ID   8FCS