Curated repository for small angle scattering data and models


SASBDB currently contains:


4370

experimental data sets

5703

models
536 experimental data sets on hold
635 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDU62 – Cereblon-midi (CRBNmidi), an engineered Cereblon construct for crystallographic and biophysical studies, bound to Mezigdomide

Cereblon-midimezigdomide experimental SAS data
PYMOL model
Sample: Cereblon-midi monomer, 37 kDa protein
Mezigdomide monomer, 1 kDa synthetic construct
Buffer: 20 mM Hepes, 500 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2023 Mar 1
Design of a Cereblon construct for crystallographic and biophysical studies of protein degraders Nature Communications 15(1) (2024)
Kroupova A, Spiteri V, Rutter Z, Furihata H, Darren D, Ramachandran S, Chakraborti S, Haubrich K, Pethe J, Gonzales D, Wijaya A, Rodriguez-Rios M, Sturbaut M, Lynch D, Farnaby W, Nakasone M, Zollman D, Ciulli A
RgGuinier 2.3 nm
Dmax 7.3 nm
VolumePorod 60 nm3

Ternary complex consisted of Human Neutrophil elastase tetramer and S.aureus Protein map Eap4-domain-4 (Δ347-476Δ) (Complex: NE/Eap4/NE)
Neutrophil elastaseProtein map experimental SAS data
S. aureus Eap is a polyvalent inhibitor of neutrophil serine proteases. J Biol Chem 2024 Aug 3;300(9):107627
J Biol Chem 300(9):107627 (2024 Aug 3)
BTB domain of longitudinals lacking (LOLA) protein
Longitudinals lacking protein, isoform G experimental SAS data
The Arthropoda-specific Tramtrack group BTB protein domains use previously unknown interface to form hexamers. Elife 2024 Sep 2;13
Elife 13 (2024 Sep 2)
mRNA capping enzyme MTase
Virus termination factor small subunitmRNA-capping enzyme catalytic subunit experimental SAS data
Structural basis of the monkeypox virus mRNA cap N7 methyltransferase complex. Emerg Microbes Infect 2024 Dec;13(1):2369193
Emerg Microbes Infect 13(1):2369193 (2024 Dec)
Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii-remeasured after storage)
Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 2024 Sep 14 33(10)
Protein Science 33(10) (2024 Sep 14)
Glucose-regulated protein 78 nucleotide-binding domain (GRP78-NBD) in complex with the C-terminal of Cerebral dopamine neurotrophic factor (C-CDNF)
Endoplasmic reticulum chaperone BiPC-terminal of the Cerebral dopamine neurotrophic factor experimental SAS data
Structural basis of CDNF interaction with the UPR regulator GRP78. Nat Commun 2024 Sep 18;15(1):8175
Nat Commun 15(1):8175 (2024 Sep 18)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.