A mechanism for histone chaperoning activity of nucleoplasmin: thermodynamic and structural models.

Taneva SG, Bañuelos S, Falces J, Arregi I, Muga A, Konarev PV, Svergun DI, Velázquez-Campoy A, Urbaneja MA, J Mol Biol 393(2):448-63 (2009) Europe PMC

SASDA55 – Nucleoplasmin

Nucleoplasmin
MWexperimental 130 kDa
MWexpected 110 kDa
VPorod 210 nm3
log I(s) 5.01×10-1 5.01×10-2 5.01×10-3 5.01×10-4
Nucleoplasmin small angle scattering data  s, nm-1
ln I(s)
Nucleoplasmin Guinier plot ln 5.01×10-1 Rg: 4.0 nm 0 (4.0 nm)-2 s2
(sRg)2I(s)/I(0)
Nucleoplasmin Kratky plot 1.104 0 √3 sRg
p(r)
Nucleoplasmin pair distance distribution function Rg: 4.0 nm 0 Dmax: 12.6 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Nucleoplasmin BUNCH model
log I(s)
 s, nm-1
Nucleoplasmin DAMMIN model
Synchrotron SAXS data from solutions of Nucleoplasmin in 20 mM Pipes buffer 150 mM NaCl, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 1M-W detector at a sample-detector distance of 2.7 m and at a wavelength of λ = 0.15 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 10 mg/ml were measured at 10°C. Four successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

Tags: X33
Nucleoplasmin (NP)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Pentamer
Mon. MW   22 kDa
Sequence   FASTA