Analysis of self-assembly of S-layer protein slp-B53 from Lysinibacillus sphaericus.

Liu J, Falke S, Drobot B, Oberthuer D, Kikhney A, Guenther T, Fahmy K, Svergun D, Betzel C, Raff J, Eur Biophys J 46(1):77-89 (2017) Europe PMC

SASDA59 – slp-B53 with Ca2+

S-layer protein
MWexperimental 222 kDa
MWexpected 116 kDa
VPorod 609 nm3
log I(s) 1.42×104 1.42×103 1.42×102 1.42×101
S-layer protein small angle scattering data  s, nm-1
ln I(s)
S-layer protein Guinier plot ln 1.42×104 Rg: 6.4 nm 0 (6.4 nm)-2 s2
(sRg)2I(s)/I(0)
S-layer protein Kratky plot 1.104 0 √3 sRg
p(r)
S-layer protein pair distance distribution function Rg: 6.8 nm 0 Dmax: 28.1 nm

Data validation

Fits and models

log I(s)
 s, nm-1
S-layer protein DAMMIF model
log I(s)
 s, nm-1
S-layer protein SASREF MX model
S-layer protein SASREF MX model
Synchrotron SAXS data from solutions of slp-B53 with Ca2+ in Water with Ca2+ were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.12 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1 and 7.5 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Slp-B53 with Ca

S-layer protein (Slp1)
Mol. type   Protein
Organism   Lysinibacillus sphaericus
Olig. state   Monomer
Mon. MW   116.0 kDa
 
UniProt   M4N8T6
Sequence   FASTA