Finci LI,
Krüger N,
Sun X,
Zhang J,
Chegkazi M,
Wu Y,
Schenk G,
Mertens HDT,
Svergun DI,
Zhang Y,
Wang JH,
Meijers R,
Neuron
83(4):839-849
(2014)
Europe PMC
Synchrotron SAXS
data from solutions of
NetrinVIV DCC56 complex
in
25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH 7
were collected
on the
EMBL P12 beam line
at the PETRA III storage ring
(DESY; Hamburg, Germany)
using a Pilatus 2M detector
at a sample-detector distance of 3.1 m and
at a wavelength of λ = 0.12 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 0.3 and 2.9 mg/ml were measured
at 10°C.
20 successive
0.050 second frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
s, nm-1
