Characterization of Der p 21, a new important allergen derived from the gut of house dust mites.

Weghofer M, Dall'Antonia Y, Grote M, Stöcklinger A, Kneidinger M, Balic N, Krauth MT, Fernández-Caldas E, Thomas WR, van Hage M, Vieths S, Spitzauer S, Horak F, Svergun DI, Konarev PV, Valent P, Thalhamer J, Keller W, Valenta R, Vrtala S, Allergy 63(6):758-67 (2008) Europe PMC

SASDA94 – Der p21

Der p21, allegren
MWexperimental 26 kDa
MWexpected 26 kDa
VPorod 50 nm3
log I(s) 9.81×101 9.81×100 9.81×10-1 9.81×10-2
Der p21, allegren small angle scattering data  s, nm-1
ln I(s)
Der p21, allegren Guinier plot ln 9.81×101 Rg: 2.7 nm 0 (2.7 nm)-2 s2
(sRg)2I(s)/I(0)
Der p21, allegren Kratky plot 1.104 0 √3 sRg
p(r)
Der p21, allegren pair distance distribution function Rg: 2.8 nm 0 Dmax: 8.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Der p21, allegren DAMMIN model
log I(s)
 s, nm-1
Der p21, allegren BUNCH model
Synchrotron SAXS data from solutions of Der p21 in 10 mM Hepes , pH 7 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 4.70 mg/ml was measured at 10°C. Two successive 120 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN

Tags: X33
Der p21, allegren (Derp21)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Dimer
Mon. MW   13 kDa
Sequence   FASTA