The Vibrio cholerae colonization factor GbpA possesses a modular structure that governs binding to different host surfaces.

Wong E, Vaaje-Kolstad G, Ghosh A, Hurtado-Guerrero R, Konarev PV, Ibrahim AF, Svergun DI, Eijsink VG, Chatterjee NS, van Aalten DM, PLoS Pathog 8(1):e1002373 (2012) Europe PMC

SASDAB4 – Truncated GbpA

Truncated GbpA
MWexperimental 46 kDa
MWexpected 44 kDa
VPorod 80 nm3
log I(s) 1.69×102 1.69×101 1.69×100 1.69×10-1
Truncated GbpA small angle scattering data  s, nm-1
ln I(s)
Truncated GbpA Guinier plot ln 1.69×102 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Truncated GbpA Kratky plot 1.104 0 √3 sRg
p(r)
Truncated GbpA pair distance distribution function Rg: 3.7 nm 0 Dmax: 12.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Truncated GbpA GASBOR model
log I(s)
 s, nm-1
Truncated GbpA SASREF model
Synchrotron SAXS data from solutions of Truncated GbpA in 25 mM Tris/HCl 150 mM NaCl, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a MAR 345 Image Plate detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 2.00 mg/ml was measured at 10°C. One 120 second frame was collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN

Tags: X33
Truncated GbpA (GbpA_truncated)
Mol. type   Protein
Organism   Vibrio cholerae
Olig. state   Monomer
Mon. MW   44 kDa
Sequence   FASTA