Recognition of nucleoplasmin by its nuclear transport receptor importin α/β: insights into a complete import complex.

Falces J, Arregi I, Konarev PV, Urbaneja MA, Svergun DI, Taneva SG, Bañuelos S, Biochemistry 49(45):9756-69 (2010) Europe PMC

SASDAC5 – ImportinA_ImportinB

ImportinA_ImportinB
MWexperimental 185 kDa
MWexpected 160 kDa
VPorod 390 nm3
log I(s) 8.00×101 8.00×100 8.00×10-1 8.00×10-2
ImportinA_ImportinB small angle scattering data  s, nm-1
ln I(s)
ImportinA_ImportinB Guinier plot ln 8×101 Rg: 5.7 nm 0 (5.7 nm)-2 s2
(sRg)2I(s)/I(0)
ImportinA_ImportinB Kratky plot 1.104 0 √3 sRg
p(r)
ImportinA_ImportinB pair distance distribution function Rg: 5.7 nm 0 Dmax: 19 nm

Data validation

Fits and models

log I(s)
 s, nm-1
ImportinA_ImportinB DAMMIF model
log I(s)
 s, nm-1
ImportinA_ImportinB SASREF model
Synchrotron SAXS data from solutions of ImportinA_ImportinB in 50 mM Tris-HCL 150 mM NaCl 1.0 mM DTT, pH 7.5 were collected on the EMBL X33 beam line at the DORIS III, DESY storage ring (Hamburg, Germany) using a Pilatus 2M detector (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 0.5 and 1 mg/ml were measured at 10°C. Eight successive 15 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Wavelength = UNKNOWN. Sample detector distance = UNKNOWN

Tags: X33
ImportinA_ImportinB (ImpA_ImpB)
Mol. type   Protein
Organism   Escherichia coli
Olig. state   Monomer
Mon. MW   160 kDa
Sequence   FASTA