| MWI(0) | 125 | kDa |
| MWexpected | 120 | kDa |
| VPorod | 240 | nm3 |
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log I(s)
4.29×103
4.29×102
4.29×101
4.29×100
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s, nm-1
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Crystal structure of the Varkud satellite ribozyme.
Suslov NB, DasGupta S, Huang H, Fuller JR, Lilley DM, Rice PA, Piccirilli JA, Nat Chem Biol 11(11):840-6 (2015) Europe PMCSASDAC9 – Varkud Satellite (VS) ribozyme in Tris
Varkud Satellite (VS) ribozyme|
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Fits and models
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Synchrotron SAXS
data from solutions of
Varkud Satellite (VS) ribozyme in Tris
in
10 mM Tris 25 mM KCl 5 mM MgCl2, pH 7.5
were collected
on the
12.3.1 (SIBYLS) beam line
at the Advanced Light Source (ALS) storage ring
(Berkeley, CA, USA)
using a Pilatus3 X 2M detector
at a wavelength of λ = 0.103 nm
(I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle).
Solute concentrations ranging between 1.5 and 4.5 mg/ml were measured
.
24 successive
frames were collected.
The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.
The low angle data collected at lower concentrations were extrapolated to infinite dilution and merged with the higher concentration data to yield the final composite scattering curve.
SAXS data from a catalytically inactive construct support the overall conformation and dimeric state of the VS ribozyme in its x-ray crystal structure. |
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